cuvette for Fluorometer to minimize multiple scattering/inner filter effects

[Drache]

I have a reduced volume quartz cuvette with the path lenght in one
direction of 10 mm and the path length in the other direction reduced.

For fluorescence measurment, can this kind of reduced-volume cuvette
cause multiple scattering or inner filter effects? I mean compared
with regular 10mm-path-length cuvette.

Thanks

[David Stone]

Post by Drache
I have a reduced volume quartz cuvette with the path lenght in one
direction of 10 mm and the path length in the other direction reduced.
For fluorescence measurment, can this kind of reduced-volume cuvette
cause multiple scattering or inner filter effects? I mean compared
with regular 10mm-path-length cuvette.

I can't see any reason why the shorter path length in one direction
would cause inner filter effects, unless you deliberately
increased the sample concentration to compensate for the path length
reduction.

As for scatter, I'm assuming that you are not deliberately scanning
the emission wavelength range across the excitation wavelength, so
any scatter from the quartz/air interfaces would be immaterial.
Again, unless you are drastically changing concentration to compensate
for the different path length in one direction, I really don't see
that there would be any effect on scatter from the solution.